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Technical Bulletins

Below is a list of technical bulletins on specific topics and applications from BD Biosciences.


BD BioCoat™ and BD Falcon™ Drug Discovery & HTS
BD BioCoat™ HTS Caco-2 Assay System
BD BioCoat™ Osteologic™ Bone Cell Culture System
BD Falcon™ FluoroBlok™ Cell Culture Insert
BD Falcon™ HTS Multiwell™ Insert System, 24-well
BD Falcon™ and BD BioCoat™ 96-well Assay Plates
BD Falcon™ Cell Culture Inserts
BD Gentest™ High Throughput Screening for P450 Inhibition
BD Matrigel™ Matrix
BD™ Extracellular Matrix (ECM) Proteins and BD BioCoat™ Cellware
BD™ Heparin Binding Plate
BD™ Oxygen Biosensor System

BD BioCoat™ and BD Falcon™ Drug Discovery & HTS
Download
435
BD Falcon™ and BD BioCoat™ Microplate Measurements and Plate Characteristics
435-A*
BD Falcon™ Microplate Measurements and Plate Characteristics
454
BD BioCoat™ Plates for High Throughput Screening (HTS)

BD BioCoat™ HTS Caco-2 Assay System
Download
415
Use of Automation-Compatible 24-well Insert System for Various Cell-Based Assays
416
Comparison of the BD BioCoat™ Intestinal Epithelial Environment with the Conventional 21-day Caco-2 System
418
Use of the 3-Day BD BioCoat™ HTS Caco-2 Assay System for Compound Permeability Measurements
426
Morphological Comparison of Caco-2 Cells in the BD BioCoat™ Intestinal Epithelial Cell Environment and the Traditional 21-Day Caco-2 Culture System

BD BioCoat™ Osteologic™ Bone Cell Culture System
Download
444
von Kossa Staining of Osteoclast Resorption on BD BioCoat™ Osteologic™ Discs
445
Tartrate Resistant Acid Phosphatase (TRAP) Staining of Osteoclasts on BD BioCoat™ Osteologic™ Discs and MultiTest Slides
446
Chondrocyte Cell Culture and Tissue Engineering on BD BioCoat™ Osteologic™ Discs and BD™ 3D Calcium Phosphate Scaffolds

BD™ Extracellular Matrix (ECM) Proteins and BD BioCoat™ Cellware
Download
417
Optimization of Cell Culture Systems that Support Growth or Differentiation of Endothelial Cells
420
Effects of ECM on Cell Proliferation and Differentiation of Hepatocytes
421
Effects of ECM Proteins on Barrier Formation in Caco-2 Cells
424
Extracellular Matrix Proteins Influence Cell Morphology and Function in Rat Neural Cultures
425
Phenotypic Modulation of Aortic Smooth Muscle Cells Using Optimized Cell Culture Environments

BD Falcon™ FluoroBlok™ Cell Culture Insert
Download
435
BD Falcon™ and BD BioCoat™ Microplate Measurements and Plate Characteristics
436
Set Up Guidelines and Dimensional Templates for Fluorescence Plate Readers Used With BD Falcon™ HTS FluoroBlok™ Insert Systems and BD BioCoat™ Multiwell Insert Cell-Based Assays
441
Screening of Anti-Metastatic Compounds Using ZsGreen1 Reef Coral Fluorescent Protein (RCFP) Labeled HT-1080 Tumor Cells
442
Screening of Anti-Metastatic Compounds by a Fluorescence Based Tumor Cell Invasion Assay
450
BD Falcon™ HTS FluoroBlok™ 96-Multiwell Insert System Enhances High-Throughput Analysis of Cell-Based Assays
451
Compatible Fluorophores and Dyes for BD Falcon™ Fluoroblok™ Inserts and Insert Systems
457
Optimized Chemotaxis Conditions for Primary Blood Monocytes or THP-1 Cells Using BD Falcon™ FluoroBlok™ 96-Multiwell Insert Plates

BD Falcon™ HTS Multiwell™ Insert System, 24-well
Download
415
Use of Automation-Compatible 24-well Insert System for Various Cell-Based Assays

BD Falcon™ and BD BioCoat™ 96-well Assay Plates
Download
423
Survey Study on Dimensions of 96-well Assay Plates Relevant to HTS Applications

BD Falcon™ Cell Culture Inserts
Download
401
Maintenance and Functional Properties of Primary Turtle Bladder Epithelial Cells Cultured on BD Falcon™ Cell Culture Inserts.
402
BD Falcon™ Cell Culture Inserts as a Support Substrate for an In Vitro Extracellular Matrix System
404
Gamma-Glutamyl Transpeptidase Assay: An Example of a Protocol for Determining the Sidedness of Asymmetrical Expression of a Membrane Protein, Enzyme, or Transport Activity in an Epithelial or Other Cell Type
405
Preparation of BD Falcon™ Cell Culture Inserts for Scanning Electron Microscopy
406
Preparation of BD Falcon™ Cell Culture Inserts for Transmission Electron Microscopy
407
An In Vitro Assay for the Study of Neutrophil Migration Through Interstitial Matrix Using BD Falcon™ Cell Culture Inserts
408
Induction of Lymphoproliferation by Antigen-Primed Macrophages Across The BD Falcon™ Cell Culture Insert
409
Use of BD Falcon™ Cell Culture Inserts to Reconstruct a Differentiated Human Epidermis In Vitro: Expression of Cell Adhesion Molecules (Integrins)
412
A Physiological and Morphological In Vitro Model For Normal Human Urothelium Cultured on BD Falcon™ Cell Culture Inserts
413
In Vitro Study of Cytokine-Mediated Activation of Endothelial Cell Permeability Using BD Falcon™ Cell Culture Inserts
459
Preparation of BD Falcon™ Cell Culture Inserts for Confocal Indirect Immunofluorescence: Fixation and Staining of Caco-2/bbe (C2) Cells with Various Dyes
463
Use of BD Falcon™ Cell Culture Inserts to Evaluate Allelopathic Effects Among Marine Phytoplankters In Vitro

BD Gentest™ High Throughput Screening for P450 Inhibition
Download
TB
A High Throughput Method for Measuring Cytochrome P450 Inhibition

BD Matrigel™ Matrix
Download
455
Methods for Implantation of BD Matrigel™ Matrix into Mice and Tissue Fixation

BD™ Heparin Binding Plate
Download
462
Heparin Preparations Immobilized to the BD™ Heparin Binding Plate

BD™ Oxygen Biosensor System
Download
438
Use of the BD™ Oxygen Biosensor System to Determine Doubling Time
443
Calculating Oxygen Concentration from Fluorescence Data on the BD™ Oxygen Biosensor System
447
Monitoring Adherent Cell Proliferation on BD™ Oxygen Biosensor Systems
448
BD™ Oxygen Biosensor System Data Collection and Normalization
449
BD™ Oxygen Biosensor System vs. Existing Methods of Measuring Toxicology

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